Isolation of host-dependent and nonparasitic mutants of the facultative parasitic Bdellovibrio UKi2.
نویسندگان
چکیده
Obligate host-dependent and nonparasitic mutants were isolated from a facultative parasitic Bdellovibrio strain. Thus it is possible to separate host-dependency from the ability to parasitize in bdellovibrios.
منابع مشابه
Ultrastructure and cell division of a facultatively parasitic strain of Bdellovibrio bacteriovorus.
Some aspects of cell development and division of Bdellovibrio bacteriovorus strain UKi2 were examined by use of electron microscopic techniques. Under saprophytic and parasitic conditions of growth, the comma-shaped cells enlarge, elongate, and form helical filaments. The mechanism of division appears to consist of an asymmetrical constriction of the filamentous cell by the cytoplasmic membrane...
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A variety of temperature-sensitive mutants of host-dependent Bdellovibrio bacteriovorus 109D were selected after ethyl methane sulfonate mutagenesis. Mutants that demonstrated plaque-forming ability reversion frequencies of 10(-8) to 10(-9) were chosen for further study. Representatives of these mutants were then characterized by phase-contrast and electron microscopy, temperature-shifted one-s...
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The major phospholipids of two strains of Bdellovibrio bacteriovorus were characterized. Both strain UKi1, which is obligately saprophytic, and strain UKi2, which is facultatively parasitic, contained phosphatidylethanolamine and phosphatidylglycerol as their major glycerophosphatides. A branched, 15-carbon fatty acid is the major component of these alkali-labile lipids. Absent from UKi1 but pr...
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A reliable method has been developed for the isolation of host-independent (H-I; i.e., "saprophytic") strains of Bdellovibrio from host-dependent (H-D; i.e., "parasitic") cultures. The technique involves growing streptomycin-resistant (Sm(r)) H-D cultures on streptomycin-susceptible (Sm(8)) host cells. A lysate containing large numbers of the Sm(r) H-D cells and some remaining Sm(8) host cells ...
متن کاملAntigenicity of bdellovibrios.
Antigenic relationships between 12 locally isolated bdellovibrios and 3 established reference strains (109D, 6-5-S, and UKi2) were investigated. Antigenicity of the strains was examined by use of the micro-complement fixation test, the serum and complement bactericidal test, and the immunodiffusion test. Antisera were prepared against one of the local strains (MS7) and against one of the establ...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 119 2 شماره
صفحات -
تاریخ انتشار 1974